p38MAPK在H9N2亚型猪流感病毒诱导小鼠急性肺损伤中的表达

doi:10.11843/j.issn.0366-6964.2014.03.018

Abstract

Abstract:

To explore the expression of p38MAPK on H9N2-SIV induced acute lung injury(ALI) in mice，180 seven weeks old SPF BALB/c female mice were randomly divided into H9N2-SIV infected group(infected group)，H9N2-SIV infected +SB203580 group(SB group) and control group，and clinical observation，pathological histology observation of lung，and the determination of distribution and expression of phospho-p38MAPK in lung were conducted on the 2^nd，4^th，6^th,day and the 8^th day post infection(p.i.) respectively. We found that the mice of infection group and SB group showed clinically apparent symptoms，compared with control group，the expression of phospho-p38MAPK protein in infection group was stronger with significant difference(P<0.05) on the 2^nd day p.i.，it reached to the top with extremely significant difference(P<0.01) on the 6^th day p.i. SB203580 can inhibits phosphorylated p38MAPK in lung tissue，lung injury of SB group mice is less than that of infection group. It indicates that p38MAPK can be activated by H9N2-SIV and participate in the occurrence and development of ALI，SB203580 can lighten pathological injury of lung in ALI.

CLC Number:

S855.3

WEI Dong，ZHANG Rui-hua，XU Ming-ju，LIU Bao-jian，WANG Guo-hua，XU Tong. Expression of p38MAPK on H9N2-SIV Induced Acute Lung Injury in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 471-476.

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References

［1］POWELL C S，WRIGHT M M，JACKSON R M. p38MAPK and MEK1/2 inhibition contribute to cellular oxidant injury after hypoxia［J］. Am J Physiol Lung Cell Mol Physiol，2004，286(4)：L826-833.
［2］ONO K，HAN J. The p38 signal transduction pathway：activation and function［J］. Cell Signal，2000，12(1)：1-13.
［3］PEIFER C，WAGNER G，LAUFER S. New approaches to the treatment of inflammatory disorders small molecule inhibitors of p38 MAP kinase［J］. Curr Top Med Chem，2006，6(2)：113-149.
［4］刘道利.p38MAPK抑制剂SB203580在重症急性胰腺炎大鼠模型中作用机制的研究［D］. 四川：泸州医学院，2010.
［5］魏东，刘英，徐彤.H9N2亚型猪流感病毒感染BALB/c小鼠动物模型的建立［J］.中国实验动物学报，2012，20(5)：54-57，8.
［6］NICK J A，YOUNG S K，BROWN K K，et al. Role of p38 mitogen-activated protein kinase in a murine model of pulmonary inflammation ［J］. J Immunol，2000，164(4):2151-2159.
［7］王存连，魏东，徐明举，等.p38MAPK信号通路在猪源H9N2流感病毒导致小鼠肺损中作用的研究［J］. 畜牧兽医学报，2014，45(2)：281-288 .
［8］NICK J A，YOUNG S K，ARNDT P G，et al. Selective suppression of neutrophil accumulation in ongoing pulmonary inflammation by systemic inhibition of p38 mitogen-activated protein kinase ［J］. J Immunol，2002，169(9):5260-5269.
［9］CHEN X L，XIA Z F，YU Y X，et al. p38 mitogen-activated protein kinase inhibition attenuates burn-induced liver injury in rats［J］. Burns,2005，31(3)：320-330.
［10］KAMINSKA B. MAPK signalling pathways as molecular targets for anti-inflammatory therapy-from molecular mechanisms to therapeutic benefits［J］.Biochim Biophys Acta，2005，1754(1-2):253-262.
［11］FUJISE T，LWAKIRI R，WU B，et al. Apoptotic pathway in the rat small intestinal mucosa is different between fasting and ischemia-reperfusion［J］.Am J Physiol Gastrointest Liver Physiol，2006，291(1)：G110-116.
［12］WIDMANN C，GIBSON S，JARPE M B，et al. Mitogen-activated protein kinase：conservation of a three-kinase module from yeast to human ［J］.Physiol Rev，1999，79(1):143-180.

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Expression of p38MAPK on H9N2-SIV Induced Acute Lung Injury in Mice

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